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Zhijing Lin, Anhui Medical University, China Updates Copyright 2023 Mallah, Soomro, Ali, Noreen, Khatoon, Kafle, Feng, Wang, Naveed and Zhang

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Roll and cook Place a triangle shaped yufka dough on a surface, with the wide side facing you

The 2xStrep-tag II-6xHis-Xpress epitope-linked RfA-cassette was amplified using as template pTK249, a pEXP1-Dest (Invtirogen) derivative encoding a 2xStrep-tag II-6xHis-Xpress epitope-linked RfA-cassette (see Supplementary Methods) and primers Strep-5-PciNdePac and Exp1-Dest-3-PspAvr

( 2 ) Additional format requirements for toppings if the amount of the topping included on the basic preparation of the menu item decreases based on the total number of toppings ordered for the menu item (such as is sometimes the case with pizza toppings)